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Updated: Aug 29, 2026
07:23
Bergmeyer Glucose Quantification for Microbiological Samples
Published on: January 17, 2025
Quantitative analysis of human salivary glucose by gas chromatography-mass spectrometry
Maria Luisa Di Gioia1, Antonella Leggio, Adolfo Le Pera
1Dipartimento di Scienze Farmaceutiche, Università degli Studi della Calabria, Arcavacata di Rende, Italy.
Abstract:
A reference analytic methodology was developed for the determination of human salivary glucose concentration. The technique involves the glucose derivatization with acetic anhydride and subsequent analysis of glucose penta-acetylated by gas chromatography combined with mass spectrometry. Glucose concentration in the biological fluid depends on the physiological status of the donor.
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01:14Gas Chromatography–Mass Spectrometry (GC–MS)
Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
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01:21Mass Spectrometry: Complex Analysis
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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