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Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
Published on: October 1, 2016
Free galactose concentrations in fresh and stored apples (Malus domestica) and processed apple products
Christine H Scaman1, Vickie Jin Wai Jim, Carol Hartnett
1Food, Nutrition, and Health, 6650 Northwest Marine Drive, University of British Columbia, Vancouver, V6T 1Z4 British Colombia, Canada. cscaman@interchange.ubc.ca
Abstract:
Gas chromatography was used to quantitate free galactose in Braeburn, Fuji, Red Delicious, and Spartan apples during cold storage, after thermal processing of apple slices and in juice produced using clarification and/or liquifaction enzymes. Spartan had significantly higher galactose levels as compared to Red Delicious apples, but changes in galactose in all varieties during 9 months of cold storage were insignificant. Blanching and canning decreased galactose levels, but doubling the thermal processing during canning increased the free galactose concentration detected in plant tissue. An enzymatic liquefaction aid used to prepare apple juice dramatically increased the free galactose content while a clarification aid caused only a slight increase due to its selective action on soluble pectin. These findings provide useful information for dietitians to base diet recommendations for galactosemic patients.

