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Proteomic analysis of diet-induced hypercholesterolemic mice
Ji Young Park1, Je Kyung Seong, Young-Ki Paik
1Yonsei Proteome Research Center, Department of Biochemistry and Bioproducts Research Center, Yonsei University, Seoul, Korea.
Proteomics
|February 5, 2004
Summary
Proteomic analysis reveals distinct liver protein changes in atherosclerosis-susceptible (B6) and resistant (C3H) mice fed an atherogenic diet. These differences in oxidative stress and lipid metabolism proteins explain their varying susceptibility to atherosclerosis.
Area of Science:
- Biochemistry
- Proteomics
- Animal Models
Background:
- Atherosclerosis susceptibility varies between mouse strains.
- Dietary factors significantly influence lipid metabolism and disease progression.
Purpose of the Study:
- To investigate differential liver protein expression in C57BL/6J (atherosclerosis-susceptible) and C3H/HeJ (atherosclerosis-resistant) mice on an atherogenic diet.
- To identify key proteins involved in the differential response to diet-induced atherosclerosis.
Main Methods:
- Proteomic analysis of liver tissues from mice fed control or high-fat atherogenic diets for eight weeks.
- Histopathological examination for lipid droplet accumulation.
- Western blotting to confirm differential protein expression.
Main Results:
- Significant alterations in plasma lipid profiles were observed between the two strains on the atherogenic diet.
- Proteomic analysis identified 30 significantly changed proteins in the liver, with 14 unique to B6 mice and 16 common to both.
- Strain-specific protein expression patterns related to oxidative stress and lipid metabolism were identified.
Conclusions:
- Distinct proteomic profiles in the liver of B6 and C3H mice correlate with their differential susceptibility to diet-induced atherosclerosis.
- Oxidative stress and lipid metabolism proteins play a crucial role in mediating these strain-specific responses.