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Updated: Aug 29, 2026
Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR
Published on: February 4, 2018
[Study on ribotyping of Lyme borreliosis spirochete in Guizhou province]
Ding-ming Wang1, Qin Hao, Xing-he Cai
1Guizhou Center for Disease Control and Prevention, Guiyang 550004, China.
Objective:
To define the main genotypes in Guizhou agricultural areas by molecular epidemiologic investigation of 21 Borrelia burgdorferi sensu lato of Lyme disease spirochetes and to provide the scientific bases for formulating a preventive policy.
Methods:
Polymerase chain reaction (PCR) technique was used to amplify the 23S(rrl)-5S(rrf) intergenic spacer, and amplified products were analyzed by restriction fragment length polymorphism (RFLP) and nucleotide sequencing.
Results:
There were two genospecies in the strains: 20 strains belong to Borrelia valaisiana, 1 strain is Borelia sp.
Conclusion:
Borrelia valaisiana was the main genotype in Guizhou agricultural areas. The harmness of B. valaisiana to human being has been confirmed. In order to efficiently prevent the harmness of agent to the people in Guizhou agriculture areas, we should study the risk further.
