Related Experiment Video
Updated: Aug 29, 2026

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
Published on: February 7, 2018
NAD(P)H oxidase-derived H(2)O(2) signals chloride channel activation in cell volume regulation and cell proliferation
Diego Varela1, Felipe Simon, Ana Riveros
1Instituto de Ciencias Biomédicas and Centro de Estudios Moleculares de la Célula Facultad de Medicina Universidad de Chile, Santiago-6530499, Santiago, Chile.
Abstract:
Cellular swelling triggers the activation of Cl(-) channels (volume-sensitive outwardly rectifying (VSOR) Cl(-) channels) in many cell types. Ensuing regulatory volume decrease has been considered the primary function of these channels. However, Cl(-) channels, which share functional properties with volume-sensitive Cl(-) channels, have been shown to be involved in other physiological processes, including cell proliferation and apoptosis, raising the question of their physiological roles and the signal transduction pathways involved in their activation. Here we report that exogenously applied H(2)O(2) elicited VSOR Cl(-) channel activation. Furthermore, activation of these channels was found to be coupled to NAD(P)H oxidase activity. Also, epidermal growth factor, known to increase H(2)O(2) production, activated Cl(-) channels with properties identical to swelling-sensitive Cl(-) channels. It is concluded that NAD(P)H oxidase-derived H(2)O(2) is the common signal transducing molecule that mediates the activation of these ubiquitously expressed anion channels under a variety of physiological conditions.
More Related Videos
07:38Functional Characterization of Na+/H+ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane
Published on: March 30, 2015
08:39Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins
Published on: May 22, 2017
Related Concept Videos
Channel Rhodopsins
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
G-Protein Gated Ion Channels
Sensory organs,...
Nitric Oxide Signaling Pathway
Concentration Cells
Consider the following voltaic cell:
Peroxisomes
Amplifying Signals via Second Messengers