Related Experiment Videos
Monoclonal antibodies that detect live salmonellae
R Torensma1, M J Visser, C J Aarsman
1Eijkman-Winkler Laboratory for Medical Microbiology, Utrecht, The Netherlands.
Applied and Environmental Microbiology
|December 1, 1992
Summary
Researchers developed nine immunoglobulin G and nine immunoglobulin M monoclonal antibodies to detect live Salmonella bacteria across multiple serogroups. These antibodies offer high specificity for distinct Salmonella serotypes, aiding in clinical isolate identification.
Area of Science:
- Immunology
- Microbiology
- Biotechnology
Background:
- Salmonella bacteria pose a significant public health risk, necessitating accurate and rapid detection methods.
- Monoclonal antibodies (mAbs) offer high specificity for microbial targets, but comprehensive panels for Salmonella serogroups are needed.
Purpose of the Study:
- To generate and characterize murine monoclonal antibodies (mAbs) against live Salmonella bacteria.
- To assess the reactivity and specificity of these mAbs against various Salmonella serogroups and serotypes.
Main Methods:
- Hybridoma technology was employed, involving fusions of immune mouse spleen cells with Sp2/0 myeloma cells.
- Nine immunoglobulin G (IgG) and nine immunoglobulin M (IgM) mAbs were produced.
- The magnetic immunoluminescence assay was utilized for antibody selection and characterization of reactivity against Salmonella serogroups (A, B, C1, C2, D, E, K) and Salmonella choleraesuis subsp. diarizonae.
Main Results:
- Eighteen mAbs (9 IgG, 9 IgM) reactive with live Salmonella were successfully generated.
- The mAbs demonstrated reactivity across multiple Salmonella serogroups, including A, B, C1, C2, D, E, and K.
- Each mAb exhibited reactivity with a distinct serotype, indicating high specificity and enabling detection of clinical isolates.
Conclusions:
- The developed panel of monoclonal antibodies provides a valuable tool for the specific detection of diverse Salmonella serogroups and serotypes.
- These mAbs show potential for accurate identification of clinical Salmonella isolates with minimal cross-reactivity to non-Salmonella bacteria.