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In vitro transfer of N,N'-diacetylchitobiose to glycoproteins in rat liver nuclei
J Frot-Coutaz1, A Degiuli, M B Martel
1Laboratoire de biochimie des membranes, UM 24, Université Claude Bernard-Lyon, Villeurbanne, France.
Abstract:
This work demonstrates that (N-acetyl[14C]glucosamine)2 is transferred from dolichyl pyrophosphate-(N-acetyl[14C]glucosamine)2 to endogenous nuclear glycoproteins. The (N-acetyl[14C]glucosamine)2 moiety is N-linked, since it can be released from the tryptic glycopeptides by N-glycosidase F and by hydrazinolysis, but not by beta-elimination. The biological significance of this direct transfer of N,N'-diacetylchitobiose to nuclear proteins remains to be elucidated.