[The inhibitory effect of 8-chloroadenosine on tumor necrosis factor-induced proto-oncogene expression of retinal
1The Third Hospital of Peking University, Peking University Eye Center, Beijing 100083, China.
Objective:
To investigate the inhibitory effect of 8-chloroadenodine (8-CLA) on tumor necrosis factor-alpha (TNF-alpha)-induced proto-oncogene expression in retinal pigment epithelial (RPE) cells.
Methods:
Primary culture and subculture of health bovine RPE cells were established in vitro. RPE cells were treated with tumor necrosis factor TNF-alpha (13.3 mmol/L) with or without 8-CLA (16 micro mol/L) for 30 minutes or 3 hours, respectively. C-fos and c-myc mRNA expression in RPE cells was detected by Northern blot analysis.
Results:
Normal RPE cells expressed c-fos and c-myc mRNA at a low level. After treatment with TNF-alpha, the expression of c-fos and c-myc mRNA was increased by 3.13 and 1.5 fold, respectively, as compared to untreated cells. 8-CLA decreased TNF-alpha-induced c-fos and c-myc mRNA expression by 25.0% and 29.0%, respectively.
Conclusions:
8-CLA can inhibit TNF-alpha-induced c-fos and c-myc mRNA expression in RPE cells. These findings may be useful for exploring new drugs for inhibiting the growth-induced proliferation of RPE.
Insights
8-chloroadenodine (8-CLA) inhibits tumor necrosis factor-alpha (TNF-alpha)-induced proto-oncogene expression in retinal pigment epithelial (RPE) cells. This suggests potential therapeutic applications for RPE-related disorders.
Area of Science:
- Cell Biology
- Molecular Biology
- Ophthalmology
Background:
- Retinal pigment epithelial (RPE) cells play a crucial role in retinal health.
- Proto-oncogene expression is implicated in cellular proliferation and disease.
- Tumor necrosis factor-alpha (TNF-alpha) is a pro-inflammatory cytokine that can induce cellular changes.
Purpose of the Study:
- To investigate the inhibitory effect of 8-chloroadenodine (8-CLA) on TNF-alpha-induced proto-oncogene expression.
- To determine the impact of 8-CLA on c-fos and c-myc mRNA levels in RPE cells stimulated by TNF-alpha.
Main Methods:
- Primary bovine RPE cells were cultured in vitro.
- Cells were treated with TNF-alpha and/or 8-CLA.
- Northern blot analysis was used to quantify c-fos and c-myc mRNA expression.
Main Results:
- TNF-alpha significantly increased c-fos and c-myc mRNA expression in RPE cells.
- 8-CLA treatment reduced TNF-alpha-induced c-fos mRNA by 25.0% and c-myc mRNA by 29.0%.
Conclusions:
- 8-CLA effectively inhibits TNF-alpha-induced proto-oncogene expression in RPE cells.
- These findings suggest 8-CLA as a potential therapeutic agent for conditions involving RPE proliferation.
