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Updated: Aug 2, 2026

Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae
Published on: February 16, 2015
Thiamine secretion in yeast
Y Haj-Ahmad1, C A Bilinski, I Russell
1Biology Department, Brock University, St. Catharines, Ont., Canada.
Abstract:
To isolate thiamine excretors and (or) overproducers, 188 cultures belonging to nine yeast and three fungal genera were screened. Nine excreted thiamine as determined by both the presence of cross-feeding zones on a thiamine-free agar medium seeded with a thiamine-requiring yeast strain and by the direct detection of excreted thiamine on agar plates. Several of these cultures produced several-fold more intracellular thiamine than the general culture population. Thiamine-requiring strains of Saccharomyces cerevisiae and S. uvarum (carlsbergensis) were identified and were tentatively assigned to 10 complementation groups.
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