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Apolipoprotein synthesis in newborn piglet intestinal explants
1Department of Pediatrics, Wyler Children's Hospital, Pritzker School of Medicine, University of Chicago, Illinois 60637.
Pediatric Research
|November 1, 1992
Summary
Epidermal growth factor (EGF) and hormones significantly impact neonatal piglet small intestine apolipoprotein synthesis. EGF boosted apolipoprotein A-I, while insulin and hydrocortisone increased apolipoprotein A-IV synthesis.
Area of Science:
- Biochemistry
- Developmental Biology
- Endocrinology
Background:
- Neonatal small intestine development involves complex regulation of lipid metabolism.
- Apolipoproteins are crucial for lipoprotein assembly and transport.
- Hormonal and growth factor influences on neonatal apolipoprotein synthesis are not fully understood.
Purpose of the Study:
- To investigate the effects of epidermal growth factor (EGF) and various hormones on apolipoprotein B-48, A-I, and A-IV synthesis.
- To elucidate the regulatory roles of EGF, glucocorticoids, and insulin in neonatal small intestinal apolipoprotein production.
Main Methods:
- Primary proximal jejunal explants from 2-day-old piglets were cultured.
- Explants were treated with EGF and hormones (hydrocortisone, insulin, thyroid hormone).
- Apolipoprotein synthesis was quantified using 35S-methionine radiolabeling and immunoprecipitation.
Main Results:
- EGF significantly increased apolipoprotein A-I synthesis, an effect blunted by hydrocortisone.
- Insulin combined with hydrocortisone significantly increased apolipoprotein A-I and A-IV synthesis (2.6-fold for A-IV).
- EGF and hydrocortisone slightly decreased apolipoprotein B-48 synthesis; thyroid hormone had no effect.
Conclusions:
- EGF, glucocorticoids, and insulin play significant regulatory roles in the developmental expression of apolipoprotein synthesis in the neonatal small intestine.
- Specific hormone and growth factor combinations differentially regulate the synthesis of key apolipoproteins during early development.