Related Experiment Videos
Sequence characterization of a sheep cDNA for antithrombin III.
R W Niessen1, A Sturk, P L Hordijk
1Center for Hemostasis, Thrombosis, Atherosclerosis and Inflammation Research, Academic Medical Center, Amsterdam, Netherlands.
Biochimica Et Biophysica Acta
|December 29, 1992
Summary
Researchers sequenced sheep liver antithrombin III (AT III) mRNA, revealing a 465-amino acid protein. The mature protein shares high sequence identity with human and bovine AT III, indicating conserved function.
Area of Science:
- Molecular Biology
- Biochemistry
- Genetics
Background:
- Antithrombin III (AT III) is a crucial huyết thanh protein involved in regulating blood coagulation.
- Understanding species-specific variations in AT III can provide insights into its structure-function relationship and evolutionary conservation.
Purpose of the Study:
- To determine the complementary DNA (cDNA) sequence of sheep liver antithrombin III (AT III) mRNA.
- To analyze the deduced amino acid sequence and compare it with homologous proteins from other species.
Main Methods:
- Isolation and sequencing of mRNA from sheep liver.
- In silico translation to determine the amino acid sequence of AT III.
- Sequence alignment and identity analysis using bioinformatics tools.
Main Results:
- The cDNA sequence encodes a sheep AT III precursor protein of 465 amino acids, including a 32-amino acid signal peptide.
- The mature sheep AT III protein exhibits high amino acid sequence identity to human (89.1%), bovine (95.6%), and rabbit (85.0%) AT III.
- Conserved cysteine residues and glycosylation sites suggest functional similarity across species, while the signal peptide shows lower interspecies identity.
Conclusions:
- Sheep antithrombin III shares significant sequence homology with its mammalian counterparts, particularly the mature protein.
- Conserved structural features imply conserved biological function in regulating coagulation across these species.
- Variations in the signal peptide may indicate species-specific differences in protein targeting or processing.