Related Experiment Videos
Selective determination, in plasma, of artemether and its major metabolite, dihydroartemisinin, by high-performance
C G Thomas1, S A Ward, G Edwards
1Department of Pharmacology and Therapeutics, University of Liverpool, UK.
Journal of Chromatography
|November 27, 1992
Abstract:
A sensitive and selective reversed-phase high-performance liquid chromatographic method for the determination of artemether and its major metabolite dihydroartemisinin in plasma has been developed. It involves extraction of plasma with dichloromethane, solid-phase separation of the two analytes and acid decomposition prior to chromatography on a C18 Spherisorb column with a mobile phase of acetonitrile-water (50:50, v/v). Run time is 30 min. The assay satisfies all of the criteria required for use in clinical pharmacokinetic studies.