Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A rapid method to isolate platelets from human blood by density gradient centrifugation.

M Hoffman1, D M Monroe, H R Roberts

  • 1Department of Pathology, University of North Carolina, Chapel Hill.

American Journal of Clinical Pathology
|November 1, 1992
PubMed
Summary

A new, simple method isolates concentrated, functionally intact platelets rapidly. This technique yields high-purity platelet preparations suitable for experimental and clinical use, even from thrombocytopenic patients.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Case of Functional, Simulating Organic Disease of the Heart.

Western journal of medicine and surgery·2024
Same author

Factor XI promotes hemostasis in factor IX-deficient mice.

Journal of thrombosis and haemostasis : JTH·2018
Same author

Harold Ross Roberts, MD, 1930-2017.

Haemophilia : the official journal of the World Federation of Hemophilia·2017
Same author

Thrombin generation and cell-dependent hypercoagulability in sickle cell disease.

Journal of thrombosis and haemostasis : JTH·2016
Same author

Future developments in the treatment of haemophilia B.

Haemophilia : the official journal of the World Federation of Hemophilia·2016
Same author

Extravascular administration of factor IX: potential for replacement therapy of canine and human hemophilia B.

Haemophilia : the official journal of the World Federation of Hemophilia·2016

Area of Science:

  • Hematology
  • Cell Biology
  • Biotechnology

Background:

  • Platelet isolation is crucial for functional studies but current methods risk activation or yield dilute preparations.
  • Existing gentle isolation techniques like density gradient centrifugation or electrophoresis are often time-consuming or result in low platelet concentrations.

Purpose of the Study:

  • To report a simple, gentle, and rapid technique for isolating concentrated platelet preparations.
  • To provide a method yielding functionally intact platelets suitable for experimental and clinical applications.

Main Methods:

  • Whole blood was layered over a density gradient medium and centrifuged.
  • The mononuclear cell layer, rich in platelets, was collected.
  • Nucleated cells were pelleted via centrifugation to isolate platelets.

Related Experiment Videos

Main Results:

  • The method achieved approximately 60% platelet recovery with less than 1% leukocyte contamination.
  • Platelet concentration was approximately 130% of the original blood concentration, with potential for higher yields.
  • About 10% of isolated platelets expressed the GMP-140 activation marker, with 70-90% expression after thrombin activation.

Conclusions:

  • This technique rapidly and easily yields functionally intact platelet preparations.
  • The method requires no specialized equipment or skills and can produce sufficient platelet concentrations for function testing, even from thrombocytopenic patients.