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Published on: March 7, 2019
Purification of S-oxynitrilase from Sorghum bicolor by immobilized metal ion affinity chromatography on different
R Woker1, B Champluvier, M R Kula
1Institut für Enzymtechnologie Heinrich-Heine-Universität Düsseldorf, Jülich, Germany.
Abstract:
The purification of the hydroxynitrile lyase (EC 4.1.2.11, S-oxynitrilase) from Sorghum bicolor is compared using different strategies. A new procedure is presented, which exploits the affinity of S-oxynitrilase towards metal ions as a key step in purification. The metal ions are immobilized by chelators on different carrier materials, e.g. Sepharose beads, microporous membranes or poly(ethylene glycol). A systematic examination demonstrates the excellent potential of immobilized metal affinity chromatography as a preparative separation method.
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