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Updated: Aug 14, 2026

Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells
Published on: June 5, 2020
[Hybridization using a digoxigenin-labeled probe for the detection of hepatitis delta virus]
L Sarría1, L Gallego, P Lardelli
1Departamento de Microbiología e Inmunología, Facultad de Medicina y Odontología, Universidad del País Vasco.
Background:
The presence of hepatitis delta virus was investigated in liver biopsies by in situ cyto-hybridization with a probe labelled with digoxigenin.
Methods:
The techniques developed included extraction of plasmid DNA by lysis by alkali, electroelution, electrophoresis in agarose gel and digoxigenin labelling, and the application in liver tissue.
Results:
Viral RNA was detected in 6 of the 10 patients, and the reactivity was exclusively restricted to the nucleolus of the hepatocytes.
Conclusion:
This method reveals as a sensitive and quick diagnostic procedure, which allows to study the intensity of the infection as well as the serological state of the patient.
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