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A mucoprotein derived from human urine which reacts with influenza, mumps, and Newcastle disease viruses

I TAMM, F L HORSFALL

    The Journal of Experimental Medicine
    |January 1, 1952
    PubMed

    Insights

    A purified mucoprotein from human urine exhibits high molecular weight and specifically binds to influenza, mumps, and Newcastle disease viruses. This interaction alters the mucoprotein, leading to a loss of its viral binding capacity.

    Area of Science:

    • Biochemistry
    • Virology
    • Immunology

    Background:

    • Normal human urine contains a mucoprotein.
    • This mucoprotein can be isolated in a highly pure form.
    • Its biological, chemical, and physicochemical properties are of interest.

    Purpose of the Study:

    • To isolate and characterize a pure mucoprotein from human urine.
    • To investigate its interaction with various viruses.
    • To understand the effects of viral interaction on the mucoprotein's properties.

    Main Methods:

    • Isolation and purification of urinary mucoprotein.
    • Characterization of molecular weight and molecular shape (axial ratio).
    • Antigenicity testing and viral reaction studies (hemagglutination inhibition).

    Main Results:

    • Isolated mucoprotein has a high molecular weight (approx. 7.0 x 10^6) and thread-like molecules (axial ratio ~100).
    • The mucoprotein is specifically antigenic and reacts strongly with influenza, mumps, and Newcastle disease viruses.
    • Viral interaction alters the mucoprotein, causing loss of viral binding capacity; inactivation kinetics resemble enzyme-substrate systems.

    Conclusions:

    • A highly pure, high molecular weight mucoprotein with specific viral reactivity has been isolated from human urine.
    • This mucoprotein's interaction with viruses leads to its inactivation, with kinetics similar to enzyme-substrate reactions.
    • While viral reactivity is lost upon alteration, other examined properties of the mucoprotein remain unchanged, though some physicochemical properties differ.
    Keywords:
    INFLUENZA VIRUSMUMPSNEWCASTLE DISEASEPROTEINS/in urine

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