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Urinary testosterone measurement by gas chromatography after solid-phase extraction and high-performance liquid
E Venturelli1, A Manzari, A Cavalleri
1National Cancer Institute, Endocrine Unit, Milan, Italy.
Journal of Chromatography
|November 6, 1992
Summary
A new method rapidly determines testosterone in urine using solid-phase extraction and chromatography. This effective technique is suitable for routine analysis, offering accurate and precise testosterone level measurements.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Endocrinology
Background:
- Accurate testosterone measurement is crucial for diagnosing various endocrine disorders.
- Existing methods for testosterone determination can be time-consuming and complex.
Purpose of the Study:
- To develop and validate a rapid, effective method for quantifying testosterone in urine.
- To establish a reliable assay suitable for routine clinical and research applications.
Main Methods:
- Solid-phase extraction (SPE) for sample clean-up.
- High-performance liquid chromatography (HPLC) for further purification.
- Gas chromatography (GC) for final testosterone determination.
Main Results:
- Mean recovery of [3H]testosterone after SPE and HPLC was 82%.
- Assay precision demonstrated a coefficient of variation of 7.9%.
- Accuracy was confirmed by standard addition and dilution assays (r2 = 0.982).
Conclusions:
- The developed SPE-HPLC-GC method provides rapid and accurate urine testosterone determination.
- The method's effectiveness and precision make it suitable for routine analysis.
- This assay can aid in the efficient diagnosis and monitoring of testosterone-related conditions.