Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Respiration in vitro: II. Electrical stimulation.

O Hamada1, E Garcia-Rill, R D Skinner

  • 1Department of Anatomy, University of Arkansas for Medical Sciences, Little Rock 72205.

Somatosensory & Motor Research
|January 1, 1992
PubMed
Summary

Electrical stimulation of the neonatal rat spinal cord can induce respiratory-like muscle activity. This finding suggests potential therapeutic applications for respiratory control even without brainstem input.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Change in EEG Activity is Associated with a Decrease in Tinnitus Awareness after rTMS.

Frontiers in neurology and neuroscience research·2021
Same author

The pedunculopontine nucleus: From posture and locomotion to neuroepigenetics.

AIMS neuroscience·2020
Same author

Focus on the pedunculopontine nucleus. Consensus review from the May 2018 brainstem society meeting in Washington, DC, USA.

Clinical neurophysiology : official journal of the International Federation of Clinical Neurophysiology·2019
Same author

Bottom-up gamma maintenance in various disorders.

Neurobiology of disease·2018
Same author

Arousal and the control of perception and movement.

Current trends in neurology·2017
Same author

Bottom-up Gamma: the Pedunculopontine Nucleus and Reticular Activating System.

Translational brain rhythmicity·2017

Area of Science:

  • Neuroscience
  • Respiratory Physiology
  • Developmental Biology

Background:

  • The neonatal rat brain stem-spinal cord preparation provides a model for studying respiratory control.
  • Spinal cord circuits are crucial for generating rhythmic motor patterns, including respiration.

Purpose of the Study:

  • To investigate the effects of electrical stimulation on intercostal muscle activity in an in vitro neonatal rat preparation.
  • To determine optimal stimulation parameters and spinal cord sites for inducing respiratory-like activity.

Main Methods:

  • Utilized a rib-attached, in vitro brain stem-spinal cord preparation from neonatal rats.
  • Applied electrical stimulation to various spinal cord segments and sites.
  • Recorded intercostal muscle activity to assess induced contractions and recruitment patterns.

Main Results:

  • Electrical stimulation induced both short-latency twitch and long-latency modulated muscle bursts, mimicking spontaneous respiratory activity.
  • A cephalocaudal recruitment order of intercostal muscles was observed, similar to spontaneous breathing.
  • Optimal stimulation involved low-frequency (0.1-0.2 Hz), long-duration (2-msec) pulses, with C1, C2, and C5 segments showing the lowest thresholds.

Conclusions:

  • Low-frequency electrical stimulation of the neonatal rat spinal cord can effectively induce coordinated, respiratory-like muscle activity.
  • This stimulation can elicit such activity even after high spinal transection, indicating independence from descending brainstem control.
  • The findings support the potential of spinal cord stimulation for restoring respiratory function.

Related Experiment Videos