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Microdetermination of G6PD isoenzyme activity in human erythrocytes by thin-layer PAG-IEF
Journal of Biochemical and Biophysical Methods
|December 1, 1992
Abstract:
An improved method of microdetermination of G6PD isoenzyme activity in human erythrocytes was developed by modification of previously reported procedures. The volume of blood samples was reduced from 2 ml to 20 microliters. After hemolysis in 2% Triton X-100 and 0.1% beta-mercaptoethanol, the samples were subjected to centrifugation and thin-layer isoelectric focusing in polyacrylamide gel (PAG-IEF). By comparison with the original method, excellent resolution was obtained by this more rapid and simple procedure.