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Cloning single-chain antibody fragments (scFv) from hybridoma cells

Lars Toleikis1, Olaf Broders, Stefan Dübel

  • 1Hospital for Women, University of Heidelberg, Germany.

Methods in Molecular Medicine
|February 13, 2004
PubMed

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Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...