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Development of rainbow trout microsatellite markers from repeat enriched libraries
C E Rexroad1, R L Coleman, A L Gustafson
1National Center for Cool and Cold Water Aquaculture, USDA-ARS, Leetown, West Virginia 25430, USA. crexroad@afrs.ars.usda.edu
Abstract:
The efficiency of developing polymorphic microsatellite markers from 2 repeat enriched libraries was evaluated. Thirty-six polymorphic microsatellite markers were developed for rainbow trout, 27 of which were informative in a mapping family. The ability of each marker to amplify genomic DNA from other salmonids was also observed.
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