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Characterization of psychrotrophic bacteria in the surface and deep-sea waters from the northwestern Pacific Ocean
O K Radjasa1, H Urakawa, K Kita-Tsukamoto
1Marine Microbiology Division, Ocean Research Institute, University of Tokyo, 1-15-1 Minamidai, Nakano-ku, Tokyo 164-8639, Japan.
Abstract:
Seventy-eight 4 degrees C-culturable bacteria were isolated using ZoBell 2216E medium from surface (0-200 m) and deep-sea (1000-9671 m) waters in the northwestern Pacific Ocean. Growth studies indicated that all 4 degrees C-culturable bacteria were psychrotrophs. Six phylotypes were observed in the surface water samples and 8 phylotypes in the deep-sea waters. Phylogenetic characterization based on 16S ribosomal DNA sequence analysis of the representative phylotypes revealed that some bacterial genera, Pseudoalteromonas, Photobacterium, and Vibrio, were common to surface and deep-sea waters, and others, Pseudomonas and Halomonas, specifically occurred in surface water. Overall, the members of Vibrionaceae appear to be dominant in both habitats.
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