Related Experiment Video
Updated: Aug 26, 2026
Culturing and Screening the Plant Parasitic Nematode Ditylenchus dipsaci
Published on: January 31, 2022
Liquid culture carbon, nitrogen and inorganic phosphate source regulate nematicidal activity by fluorescent
1Soil Biology and Biochemistry, Department of Botany, University of Karachi, Karachi 75270, Pakistan. imran_757850@yahoo.com
Aims:
The aim of the present investigation was to determine the influence of nutrients on the nematicidal activity by Pseudomonas aeruginosa strain IE-6S+ and Ps. fluorescens strain CHA0 in vitro.
Methods And Results:
Culture filtrate of IE-6S+ and CHA0 obtained from chemically defined medium caused mortality of Meloidogyne javanica juveniles in vitro and that growth medium amended with various C, N or inorganic phosphate (Pi) sources markedly influenced nematicidal activity of the two bacteria. Glycerol (C source), propionate (fatty acid precursor) and L-lysine (N source) enhanced nematicidal activity while glucose (C), L-valine (N) and Pi substantially repressed nematicidal activity of the two bacteria.
Conclusion:
Liquid culture amendments with various C, N or Pi sources modulate the biosynthesis of nematicidal agents to a different extent in vitro.
Significance And Impact Of The Study:
Developing bacterial strains more responsive to certain environmental signals can be exploited for increased secondary metabolite production in pharmaceutical fermentations and offers new avenues to improve biocontrol.
