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Related Experiment Videos

Evidence that Hy- RBCs express weak Joa antigen.

Terry L Scofield1, John P Miller, Jill R Storry

  • 1American Red Cross-North Central Blood Services, St. Paul, Minnesota 55107, USA. scofieldt@usa.redcross.org

Transfusion
|February 14, 2004
PubMed
Summary

This study reveals that Hy- blood cells can express the Joa antigen weakly, challenging previous assumptions about blood group typing. This finding improves understanding of rare blood group antigen expression.

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Area of Science:

  • Immunology
  • Transfusion Medicine
  • Genetics

Background:

  • The identification of antibodies like anti-Hy and anti-Joa is challenging due to poorly characterized reagent red blood cells (RBCs).
  • Historically, RBCs with the Hy- phenotype were typed as Gy(a+w), Hy-, Jo(a-), and those with the Jo(a-) phenotype as Gy(a+), Hy+w, Jo(a-).

Observation:

  • A case involving a 71-year-old woman identified an anti-Joa antibody in her serum.
  • This antibody reacted weakly with Hy- RBCs, contradicting established typing.
  • Adsorption and elution studies confirmed the presence of a weak Joa antigen on Hy- RBCs.

Findings:

  • The patient's RBCs were serologically determined as Gy(a+), Hy+, Jo(a-).
  • DNA analysis confirmed the patient was homozygous for the JO allele, consistent with Jo(a-) status.

Related Experiment Videos

  • This case demonstrates that Hy- RBCs can express low levels of the Joa antigen.

    • Implications:

    • Challenges existing understanding of Joa antigen expression in relation to the Hy phenotype.
    • Highlights the importance of considering weak antigen expression in blood group serology.
    • Suggests a need for re-evaluation of reagent RBC characterization for identifying rare antibodies.