Trapped water of human erythrocytes and its application in cryopreservation

The novel differential scanning calorimetry method as a technique for determining human red cell volume during freezing process has been reexamined and has been shown to provide a final erythrocyte volume to be 53% of its isotonic value after freezing from 0 to -40 degrees C. A new type of electronic particle counter (Multisizer 3, Beckman Coulter Inc., USA) was used to measure cell volume changes in response to equilibration in anisotonic media, and which gave out an equilibrated volume to be 57% of cell isotonic value in solution of 3186 mOsm. Both of these results indicate that 34-40% of intracellular water is trapped and is unavailable for participation in osmotic shifts. These findings are consistent with the published data that at least 20-32% (v/v) of the isotonic cell water is retained within RBCs. Then the application of trapped water in both simulation of freezing models and freezing-drying control was pointed out.