Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

ExQuest, a novel method for displaying quantitative gene expression from ESTs.

Aaron C Brown1, Kristin Kai, Marjorie E May

  • 1The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA.

Genomics
|February 14, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Direct Measurement of Adipose Thermogenesis by Isothermal Microcalorimetry.

Cells·2026
Same author

Thermogenesis is limited by cellular competence.

Frontiers in cell and developmental biology·2026
Same author

The Sleeping Wolf - Widefield OCT Angiography Revelations of Insidious Systemic Lupus Erythematous-Associated Retinal Vasculitis.

Retinal cases & brief reports·2026
Same author

BXSB.Yaa: A Translational Model of Toll-Like Receptor 7-Type I Interferon-Driven Systemic Lupus Erythematosus.

Immunology·2026
Same author

Quantifying Adipose Tissue Thermogenesis Using Highly Sensitive Isothermal Microcalorimetry.

bioRxiv : the preprint server for biology·2026
Same author

Isoform-Specific Control of Adipose Thermogenesis by the miR-27 Family Reveals Antagonism within a Polycistronic miRNA Cluster.

bioRxiv : the preprint server for biology·2025

ExQuest is a bioinformatics tool that organizes expressed sequence tag (EST) data to reveal gene expression patterns across mammalian tissues. This helps researchers understand gene function and identify important genes in genetic studies.

Area of Science:

  • Bioinformatics
  • Genomics
  • Molecular Biology

Background:

  • Mammalian gene expression data, particularly from expressed sequence tags (ESTs), is abundant but requires effective tools for analysis.
  • Tissue-specific cDNA libraries provide insights into gene expression levels, where EST counts correlate with mRNA abundance.

Purpose of the Study:

  • To develop an interactive bioinformatics tool, ExQuest (Expressional Quantification of ESTs), for organizing and visualizing mammalian gene expression data.
  • To enable researchers to extract and interpret gene expression patterns from public EST databases.

Main Methods:

  • ExQuest organizes the public EST database (dbEST) into hierarchical tissue classes.
  • It analyzes both mRNA and genomic sequences to report tissue or developmental gene expression patterns.

Related Experiment Videos

  • The tool generates interactive "transcriptome" maps displaying gene expression in the context of assembled chromosomes.

    • Main Results:

    • ExQuest provides organized tissue expression patterns for mammalian genes.
    • Interactive transcriptome maps visualize gene expression across chromosomes.
    • The tool facilitates the prioritization of candidate genes within mapped loci.

    Conclusions:

    • ExQuest offers a novel approach for investigating the genomic basis of gene expression.
    • The tool aids in understanding tissue-specific gene activity and developmental patterns.
    • It serves as a valuable resource for prioritizing candidate genes in genetic research.