[90-day subchronic toxicity study of aloe whole-leaf powder]

90-day subchronic toxicity study of aloe whole-leaf powder was conducted to observe the effect of aloe whole-leaf powder on health. 88 SD rats were divided randomly into 4 groups, and each group consisted of 11 males and 11 females. The animals in group 2-4 received aloe whole-leaf powder mixed in regular rodent diet at doses of 2, 4 and 8 g/kg BW (by rate of 2.5, 5 and 10 percent in diet) for 90 days. The animals in group 1 received the regular rodent diet. The results showed that aloe whole-leaf powder promoted defecation of rats. Food efficiency and body weight males exposed to 8 g/kg BW were significantly less than those of the controls. Food efficiency males exposed to 4 g/kg BW was significantly decreased, but body weight was not affected. No adverse effect were observed on food efficiency and body weight by males exposed to 2 g/kg BW and all exposed groups of females. Relative kidney weight was significnatly increased in males exposed to 8 g/kg BW and females exposed to 2 g/kg BW or greater. Relative testis weight of rats exposed to 4 and 8 g/kg BW were significantly increased compared to the control groups. Aloe whole-leaf powder at the dose of 2, 4 and 8 g/kg BW did not have adverse effect on hematology, serum AST, ALT, TC, TG, Cr as well as NAG activity of urine, serum BUN was significantly increased at the dose of 8 g/kg BW. Pathology findings: the incidences of pigmentation in renal tubular, mesenteric lymph nodes and lamina propria of the colonic mucosa, and proliferation in mesenteric lymph nodes were significantly increased at all of the exposed groups, but no pigmentation in colonic mucosa was observed at the dose of 2 g/kg BW. No pathological changes were observed in livers, spleens, testes (or ovaries). It was concluded that observed adverse effect level of aloe whole-leaf powder was 2 g/kg BW (LOAEL of aloin was 11.8 g/kg BW).