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Updated: Aug 26, 2026

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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
Published on: March 30, 2015
Real-time quantitative polymerase chain reaction (RO-PCR) using the LightCycler: a rapid, high-throughput method for
1Division of Paediatric Haematology-Oncology, Children's Medical Institute, National University Hospital, National University of Singapore, 5 Lower Kent Ridge Road, Singapore 119074.
Annals of the Academy of Medicine, Singapore
|February 19, 2004
Abstract
No abstract available in PubMed .
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Real Time RT-PCR
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
The real-time quantification of the number of amplified products is...
PCR - Polymerase Chain Reaction
Overview

