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Related Experiment Videos

Invader assay for RNA quantitation.

Marilyn C Olson1, Tsetska Takova, LuAnne Chehak

  • 1Third Wave Technologies Inc., Madison, WI, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 19, 2004
PubMed
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The Invader assay offers sensitive, quantitative detection of DNA or RNA without amplification. This nucleic acid detection method utilizes enzymatic cleavage for high specificity, even with similar gene sequences.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Nucleic acid detection is crucial for diagnostics and research.
  • Existing methods often require target amplification or reverse transcription.
  • High specificity is needed to differentiate similar genetic sequences.

Purpose of the Study:

  • To introduce and characterize the Invader assay for nucleic acid detection.
  • To demonstrate the assay's ability to detect DNA and RNA directly.
  • To highlight the specificity of the Invader assay for discriminating gene sequences.

Main Methods:

  • Utilizing a homogeneous, isothermal signal amplification system.
  • Employing Cleavase enzymes for structure-specific cleavage of hybridized oligonucleotides.

Related Experiment Videos

  • Direct detection of DNA or RNA without prior amplification or reverse transcription.

    • Main Results:

    • The Invader assay provides quantitative detection of nucleic acids.
    • Direct detection of DNA and RNA is achievable.
    • High specificity allows discrimination of single nucleotide polymorphisms and homologous mRNAs.

    Conclusions:

    • The Invader assay is a versatile tool for nucleic acid detection.
    • Its reliance on enzymatic cleavage ensures high specificity.
    • The assay is applicable to a wide range of DNA and RNA sequences.