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Updated: Aug 26, 2026
Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632
Published on: November 6, 2021
[Human gingival epithelial cells cultured by a combined method of serum containing medium and serum free medium]
Bao-Hong Zhao1, Wei Bai, Hai-Lan Feng
1Department of Prosthodontics, Peking University School of Stomatology, Beijing 100081, China.
Objective:
To search for a simple and rapid cell culture method for human gingival epithelial cells with a high success rate.
Methods:
Culture medium containing serum has been proved to have the ability of accelerating the early adhesion of human gingival tissue blocks, and the migration of gingival epithelial cells from the rim of the blocks. By means of this, we introduced the serum containing DMEM to the cell culture medium within the first 7-10 days, and changed with serum free cell culture medium to accelerate the mitosis, proliferation, and migration of the gingival epithelial cells, which had moved out from the tissue blocks. Using the combined method, cells were identified by the method of morphology, immunohistochemistry and analysis of the cell growth curve, as well as SEM. Compared study of the effects on the cell growth between combined method and serum containing DMEM or serum free EpiLife culture method was conducted.
Results:
Cells were harvested within 17-22 days. Primary culture success rate was 90.6%. Cultured cells were slabstone-shaped. Immunohistochemistry analysis of keratin antibody showed a positive result. The cells had stronger ability of migration and proliferation in the serum free cell culture medium compared with that in the serum medium.
Conclusion:
This method can culture the gingival epithelium cells conveniently with accelerated speed.
