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Updated: Aug 26, 2026

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
Published on: March 1, 2018
High-performance liquid chromatographic method with column-switching and post-column reaction for determination of
Yuji Hirowatari1, Katsuko Hara, Hiroshi Kamihata
1Scientific Instruments Division, Tosoh Corporation, Ayase, Kanagawa 252-1123, Japan. hirowata@tosoh.co.jp
Objectives:
Measurement of serotonin concentrations in blood is considered to be important for the clinical studies of diseases involving vascular endothelial injury. We have developed a high-performance liquid chromatographic (HPLC) method for measurement of serotonin level in platelet-poor plasma (PPP).
Methods:
Venous blood samples were collected using vacuum tubes containing ethylenediaminetetraacetic acid (EDTA) dipotassium salt; the optimum concentration in blood was 7.4 mmol/l. Serotonin in samples was separated by HPLC with a column-switching system, and was specifically converted into a fluorescent derivative with benzylamine for convenient detection.
Results:
The between-day assay coefficient of variation for a serotonin level (5.1 nmol/l) in PPP was 6.2%. The mean concentration of serotonin in PPP in healthy subjects was 5.7 +/- 3.0 nmol/l. The serotonin levels in PPP in patients with ischemic heart disease were significantly higher than those in healthy subjects (P = 0.001).
Conclusions:
The developed method had sufficient performance to determine serotonin levels in PPP from patients with ischemic heart disease.
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