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Related Experiment Videos

Viable infectious cell sorting in a BSL-3 facility.

Stephen P Perfetto1, David R Ambrozak, Mario Roederer

  • 1Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 21, 2004
PubMed
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Protecting flow cytometer operators is crucial when sorting infectious cells. This study details a rapid procedure using aerosol containment, physical barriers, and environmental controls to ensure operator safety during cell sorting.

Area of Science:

  • Biomedical Engineering
  • Laboratory Safety
  • Cell Biology

Background:

  • Increasing demand for high-speed cell sorting of infectious and therapeutic samples raises safety concerns for flow cytometer operators.
  • Ensuring operator protection is paramount when handling potentially hazardous biological materials during cell sorting procedures.

Purpose of the Study:

  • To describe a rapid, sensitive, and reproducible procedure for assuring sample containment during cell sorting.
  • To evaluate the effectiveness of implemented safety measures in preventing aerosol escape.

Main Methods:

  • Implementation of an aerosol management system with negative pressure and HEPA filtration.
  • Utilization of physical barriers, containment within a Biosafety Level 3 (BSL-3) laboratory, and operator respiratory protection.

Related Experiment Videos

  • Quantification of aerosol containment using fluorescent Glo-Germ particles and a vacuum air sampling system.

    • Main Results:

    • The described procedure effectively contains aerosols generated during cell sorting.
    • Microscopic examination of collected samples confirmed minimal to no aerosol escape.
    • The containment method is quick, sensitive, and reproducible.

    Conclusions:

    • The implemented safety protocol significantly reduces the risk to operators when sorting viable infectious cells.
    • This procedure provides a reliable method for validating sample containment in flow cytometry.
    • Enhanced safety measures are essential for the routine handling of infectious samples in cell sorting applications.