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Hydrogen/deuterium isotope effect in the oscillating peroxidase-oxidase reaction
1Laboratorium für Technische Chemie, Eidgenössische Technische Hochschule (ETH), Zurich, Switzerland.
Biochemical and Biophysical Research Communications
|July 31, 1992
Abstract:
The oscillatory peroxidase-oxidase reaction has been investigated by using NADH deuterated in the nicotinamide 4-A position. A considerable kinetic hydrogen/deuterium isotope effect on the oscillatory behavior was revealed, which may provide an additional valuable tool for mechanistic studies and for discriminating between various mechanistic models of the peroxidase-oxidase reaction. Particularly, this effect manifests in different oscillation frequencies. A sequence of simple and aperiodic oscillations was found between two stable steady states.