Related Experiment Videos

Isolation and sequence determination of cDNA encoding PMP-22 (PAS-II/SR13/Gas-3) of human peripheral myelin

K Hayasaka1, M Himoro, K Nanao

  • 1Department of Pediatrics and Dentistry, Akita University School of Medicine, Japan.

Biochemical and Biophysical Research Communications
|July 31, 1992
PubMed

Insights

Researchers isolated a human peripheral myelin protein 22 (PMP-22) cDNA clone. This 1823 bp clone encodes a 160-residue polypeptide highly homologous to PMP-22 in other species.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Peripheral myelin protein 22 (PMP-22) is crucial for peripheral nervous system myelination.
  • Understanding PMP-22's structure and function is key to diagnosing and treating demyelinating disorders.

Purpose of the Study:

  • To isolate and characterize the full-length cDNA of human peripheral myelin protein 22 (PMP-22).
  • To analyze the deduced amino acid sequence of human PMP-22 and compare it with homologous proteins in other species.

Main Methods:

  • Construction and screening of a human fetus spinal cord cDNA library.
  • DNA sequencing to determine the clone's length and identify the open reading frame.
  • Amino acid sequence analysis and homology comparison.

Main Results:

  • A full-length human PMP-22 cDNA clone of 1823 base pairs was successfully isolated.
  • The clone contains a 480 bp open reading frame encoding a 160-amino acid polypeptide.
  • The deduced amino acid sequence shows high homology to bovine (PAS-II), rat (SR13), and mouse (Gas-3) PMP-22.

Conclusions:

  • The isolated human PMP-22 cDNA provides a valuable resource for further functional studies.
  • The high sequence homology suggests conserved function of PMP-22 across mammalian species.
  • This finding contributes to the understanding of peripheral myelin development and associated neuropathies.

Related Concept Videos