Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Exertional heat injury and gene expression changes: a DNA microarray analysis study.

Larry A Sonna1, C Bruce Wenger, Scott Flinn

  • 1Thermal and Mountain Medicine Div., United States Army Research Institute of Environmental Medicine, Natick, MA 01760, USA. larry.sonna@na.amedd.army.mil

Journal of Applied Physiology (Bethesda, Md. : 1985)
|February 24, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dry and humid heat acclimation induces similar adaptations and cross-acclimation benefits in trained male cyclists.

The Journal of physiology·2026
Same author

Tribute to a great and unique thermal physiologist - Leo Charles Senay.

Temperature (Austin, Tex.)·2026
Same author

A Rare Metastatic Fibrous Tumor: Review of Literature With a Case Report.

JTO clinical and research reports·2025
Same author

Influence of Exercise Heat Acclimation Protocol Characteristics on Adaptation Kinetics: A Quantitative Review With Bayesian Meta-Regressions.

Comprehensive Physiology·2025
Same author

Comment on "Comparison of core temperature and mental status of two runners experiencing exertional heat stroke after a road race".

Journal of science and medicine in sport·2025
Same author

Whole body sweat rate prediction: indoor treadmill and cycle ergometer exercise.

Journal of applied physiology (Bethesda, Md. : 1985)·2024
Same journal

Thoroughbred horses susceptible to Recurrent Exertional Rhabdomyolysis have elevated skeletal muscle mitochondrial capacities.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
Same journal

Change in Neutrophil-to-Lymphocyte Ratio after acute and chronic exercise: A Systematic Review and Meta-Analysis.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
Same journal

Ankylosing spondylitis and muscle sympathetic nerve activity: a case study.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
Same journal

Intracranial vasomotor and blood flow responses to light intensity aerobic exercise in young adults: a 4D flow MRI study.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
Same journal

Comparative assessments of the COSMED adaptive mixing chamber vs. breath-by-breath methods for oxygen uptake measurements in recreationally active adults.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
Same journal

Can we assess exercise metabolism from skin? Metabolomic profiles in skin dialysate collected during exercise.

Journal of applied physiology (Bethesda, Md. : 1985)·2026
See all related articles

Exertional heat injury (EHI) triggers a wide gene expression response in blood cells, going beyond simple heat stress. This suggests EHI results from multiple interacting factors, not just heat alone.

Area of Science:

  • Molecular biology
  • Human physiology
  • Stress response

Background:

  • Exertional heat injury (EHI) is a serious condition affecting individuals during intense physical activity in hot environments.
  • Previous research has identified heat shock responses in vitro, but the in vivo response during EHI is less understood.
  • Understanding the molecular basis of EHI is crucial for developing effective prevention and treatment strategies.

Purpose of the Study:

  • To investigate gene expression changes in peripheral blood mononuclear cells (PBMCs) of individuals with EHI.
  • To compare the in vivo EHI gene expression profile with previously established in vitro heat shock responses.
  • To explore the molecular mechanisms underlying EHI and identify potential contributing factors.

Main Methods:

Related Experiment Videos

  • Collected peripheral blood mononuclear cell (PBMC) RNA from Marine recruits diagnosed with EHI and age/gender-matched controls.
  • Utilized Affymetrix arrays for gene expression analysis on samples taken at multiple time points during and after EHI.
  • Compared gene expression data from EHI patients against control samples obtained before and after a strenuous heat-based exercise ('The Crucible').

    • Main Results:

    • Identified 361 transcripts with increased expression and 331 transcripts with decreased expression (twofold or greater) in EHI patients.
    • Observed changes in heat shock proteins and non-heat shock proteins, consistent with in vitro heat shock responses.
    • Detected alterations in interferon-induced sequences and other nonspecific stress response genes not previously linked to in vitro heat shock.

    Conclusions:

    • Exertional heat injury (EHI) induces a broad molecular stress response detectable in PBMCs.
    • Heat stress alone cannot fully explain all observed gene expression changes during EHI.
    • The findings support the hypothesis that EHI arises from the cumulative impact of multiple interacting adverse stimuli.