Related Experiment Videos
Syringe-cartridge solid-phase extraction method for patulin in apple juice
Thomas A Eisele1, Midori Z Gibson
1Tree Top Inc., PO Box 248, 111 S. Railroad Ave, Selah, WA 98942, USA. teisele@treetop.com
Journal of AOAC International
|February 26, 2004
Summary
A new solid-phase extraction (SPE) method accurately determines patulin in apple juice. This technique helps ensure juice safety and compliance with regulatory standards.
Area of Science:
- Food Chemistry
- Analytical Chemistry
- Mycotoxin Analysis
Background:
- Patulin is a mycotoxin found in apple products.
- Regulatory bodies set action levels for patulin to ensure food safety.
- Accurate and efficient methods are needed for patulin determination.
Purpose of the Study:
- To develop and validate a syringe-cartridge solid-phase extraction (SPE) method for patulin determination in apple juice.
- To assess the method's applicability in an industrial quality assurance setting.
Main Methods:
- Solid-phase extraction (SPE) using a macroporous cartridge.
- Sample preparation involved conditioning, washing with sodium bicarbonate and acetic acid, and elution.
- Determination of patulin using reversed-phase liquid chromatography (RP-LC).
- Mobile phase composition: 81% acetonitrile, 9% water, 10% 0.05M potassium phosphate buffer (pH 2.4).
Main Results:
- Average recoveries of patulin were 92%.
- Relative standard deviation was 8.0% for spiked samples (50 ng/mL).
- The method is suitable for monitoring apple juice for patulin.
Conclusions:
- The developed SPE-RP-LC method is effective for patulin quantification in apple juice.
- The method meets the requirements for industrial quality assurance and regulatory compliance.
- This technique supports monitoring apple juice to comply with the FDA action level of 50 microg/kg.