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[Effects of emodin on lung fibroblast proliferation and cell cycle in vitro]
Yun Qu1, Ping Yao, Ting-qian Li
1Department of Rehabilitation, West China Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To gain a better understanding of the effect of emodin on lung fibroblast (LF) proliferation in vitro.
Methods:
The methods of cell cultured in vitro, 3H-TdR incorporation, flow cytometry and electron microscopy were used in research to compare LF proliferation, cell cycle and morphologic change of LF in vitro.
Results:
It was found that emodin reduced the proliferation of LF in vitro (P < 0.01), and such inhibitory effect was dose-dependent. Emodin delayed the progression of LF from G1 to S and G2 phase (P < 0.01). Also, electron microscopic examination demonstrated that the ultrastructure of LF in emodin group was not markedly worse as compared with that in the control group.
Conclusion:
Emodin inhibits the proliferation of LF and delays the progression of LF cell cycle. Emodin at the dose of 100 micrograms/ml does not destroy the ultrastructure of LF cells. These findings may provide a theoretical basis for the endeavors to use emodin for preventing lung fibrosis diseases.