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Solid-state NMR spectroscopy of aligned lipid bilayers at low temperatures
Dong-Kuk Lee1, Katherine Henzler Wildman, Ayyalusamy Ramamoorthy
1Department of Chemistry, Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109-1055, USA.
Abstract:
This study, for the first time, demonstrates that it is possible to mechanically align lipid bilayers at a very low temperature (as low as the gel-to-liquid crystalline phase transition temperature). Performing NMR experiments on mechanically aligned lipid bilayers at a low temperature increases the signal-to-noise ratio, the resolution, and the span of NMR parameters. The increased lifetime of the alignment and the nature of the bilayer sample would enhance the application of solid-state NMR techniques to study membrane proteins.
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