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Updated: Aug 11, 2026

An Explant Assay for Assessing Cellular Behavior of the Cranial Mesenchyme
Published on: January 20, 2013
Expression, crystallization and preliminary X-ray analysis of extracellular modules of the neural cell-adhesion
Nikolaj Kulahin1, Christina Kasper, Michael Gajhede
1Protein Laboratory, Institute of Molecular Pathology, Panum Institute, Blegdamsvej 3 C, DK-2200 Copenhagen, Denmark.
Abstract:
Recombinant proteins consisting of either the four or five amino-terminal immunoglobulin (Ig) modules of the rat neural cell-adhesion molecule NCAM or the whole extracellular part [six Ig and five fibronectin type III (F3) modules] of mouse L1 have been expressed in Drosophila S2 cells. The proteins have been purified and crystallized. The crystals of the recombinant protein containing the four amino-terminal Ig modules of NCAM diffract X-rays to approximately 4 A resolution and belong to space group P622 or P6(3)22, with unit-cell parameters a = b = 258.7, c = 182.4 A. No diffraction was observed for the other two protein constructs. This is a step towards determining the structure of multimodular constructs of cell-adhesion molecules that exhibit high structural flexibility.
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