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Updated: Aug 26, 2026
Rat Mesentery Angiogenesis Assay
Published on: June 18, 2011
[Mast cell number as an indicator of angiogenesis in autotransplanted tissue]
I V Maĭborodin1, A V Domnikov, K P Kovalevskiĭ
1Laboratory of Pathologic Morphology, Clinical Department of Scientific Research, Institute of Clinical and Experimental Morphology and Lymphology, SB RAMS, Novosibirsk.
Using the methods of light microscopy, mast cell (MC) distribution was studied and their number was counted in the dermis of autotransplanted vascularized tissue complexes at different time intervals following the operation. The number of MC in the dermis during the process of angiogenesis was closely connected with the number of blood vessels as evidenced by the direct correlation between these two parameters 2-3 weeks, 2-4 months and 5-8 months after the transplantation. MC seem to participate in the control of blood and lymph vessel formation as witnessed by high MC concentration close to the formed vessels and low MC concentration at a distance from them 2-4 and 5-8 months following the transplantation. It is suggested that the data on MC number may be helpful in prognosis of angiogenesis intensity in tissues, time intervals of edema reduction after organ or tissue transplantation, outcome of lymphedema treatment, velocity of tumor metastasis spread.
Using the methods of light microscopy, mast cell (MC) distribution was studied and their number was counted in the dermis of autotransplanted vascularized tissue complexes at different time intervals following the operation. The number of MC in the dermis during the process of angiogenesis was closely connected with the number of blood vessels as evidenced by the direct correlation between these two parameters 2-3 weeks, 2-4 months and 5-8 months after the transplantation. MC seem to participate in the control of blood and lymph vessel formation as witnessed by high MC concentration close to the formed vessels and low MC concentration at a distance from them 2-4 and 5-8 months following the transplantation. It is suggested that the data on MC number may be helpful in prognosis of angiogenesis intensity in tissues, time intervals of edema reduction after organ or tissue transplantation, outcome of lymphedema treatment, velocity of tumor metastasis spread.
