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Simulation of the kinetics of a sphere attached to a fluctuating polymer: Implications for target search by

J Chakrabarti1, S Roy

  • 1S. N. Bose National Centre for Basic Sciences, Block-JD, Sector-III, Salt Lake, Calcutta 700091, India. jaydeb@boson.bose.res.in

Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics
|March 5, 2004
PubMed

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...