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Endothelial cell seeding after carotid endarterectomy in a canine model reduces platelet uptake
A V Sterpetti1, R D Schultz, R T Bailey
1Department of Surgery, Creighton University, Omaha, Nebraska.
Insights
Endothelial cell seeding on arteries after endarterectomy significantly reduced platelet deposition. This technique shows promise for improving healing and preventing stenosis after vascular surgery.
Area of Science:
- Vascular Surgery
- Biomedical Engineering
- Regenerative Medicine
Background:
- Post-endarterectomy platelet deposition is critical for vessel healing and progressive stenosis.
- Endothelial cell seeding is a potential strategy to improve outcomes after endarterectomy.
Purpose of the Study:
- To investigate the effect of endothelial cell seeding on platelet deposition in endarterectomized arteries using a canine model.
- To assess the feasibility and efficacy of endothelial cell seeding in reducing platelet uptake.
Main Methods:
- Canine carotid arteries underwent endarterectomy and subsequent seeding with endothelial cells or sham procedure.
- Platelet deposition was quantified using 111indium-labeled autologous platelets and gamma camera imaging.
- Occlusion rates were assessed at 4 and 5 weeks post-surgery.
Main Results:
- Endothelial cell seeding significantly reduced platelet uptake on endarterectomized arteries compared to sham controls.
- Seven arteries in each group were found to be occluded at the study endpoint.
- The study demonstrated the feasibility of endothelial cell seeding in this model.
Conclusions:
- Endothelial cell seeding on endarterectomized arteries is feasible and effectively reduces platelet deposition.
- Further improvements in seeding efficiency and reduction of cell loss are needed for clinical application.
- This technique holds potential for enhancing vascular healing and preventing post-surgical stenosis.
Abstract:
Post-endarterectomy platelet deposition may play an important role not only in vessel wall healing, but also in the development of progressive stenosis. Using a canine model, we investigated the effect of endothelial cell seeding on platelet deposition on endarterectomised arteries. Thirteen dogs underwent bilateral carotid intimectomy (5 cm long) and one side was seeded with an average of 2 x 10(6) viable freshly harvested endothelial cells. Blood flow was restored 20 min after seeding. On the contralateral side, a sham-seeding was performed. Deposition of 111indium-labelled autologous platelets was studied with sequential gamma camera images 3-5 h, 1, 2, 3, 4 days and 4 weeks after surgery. Platelet uptake was statistically reduced on the seeded side. Animals were killed at 4 weeks (nine dogs) and 5 weeks (four dogs) after surgery. Seven arteries for each group were found to be occluded. We conclude that endothelial cell seeding on endarterectomised arteries is feasible and reduces platelet uptake. Improvement in the efficiency of seeding and reduction of endothelial cell loss might permit clinical application of this technique.