Changes of angiotensinogen expression in arteries of tail-suspended rats

Previous findings from our laboratory have demonstrated that simulated microgravity may result in atrophic changes with depressed vasoconstrictor responsiveness in hindquarter vessels, and hypertrophic changes with enhanced vasoconstrictor responsiveness in cerebral arteries of rats. However, the mechanisms of this differential adaptation are still not well understood. Local renin-angiotensin system (L-RAS) has been found to be actively involved in the remodeling of arteries. We hypothesized that L-RAS may function as a local regulatory mechanism in the microgravity-induced differential changes of arterial vessles. Angiotensinogen (AGT) is the only and indispensable substrate of local renin-angiotensin system (L-RAS). In the present work, the expression changes of AGT mRNA and protein level as well as its time course characteristics were examined.