Three-dimensional (3-D) structures formed by immortalized human fibroblast cells in simulated microgravity

3-D structures were obtained at rotatory cultivation of CH immortalyzed human fibroblasts attached to glass microcarrier beads. The morphology of cells from these cultures was studied by scanning electron microscopy. A number of structural alterations in fibrillar filopodia of CH cells were revealed as compared with cells grown in stationary monolayer cultures, namely, smaller length, uneven caliber, the presence of curvatures, and disturbed branching pattern. Filopodia displayed unusual formations: protuberance-like and "mammoth's tusk"-like off-shoots, foamy spreadings in distal segments, and spiral windings of filopodia. The susceptibility of CH cells morphology to mechanical environment makes them a promising model for gravitational biology studies.