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Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
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Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)

Published on: August 6, 2011

Real-time multiplex PCR assay for detection of Brucella spp., B. abortus, and B. melitensis

William S Probert1, Kimmi N Schrader, Nhi Y Khuong

  • 1Microbial Diseases Laboratory, Division of Communicable Disease Control, California Department of Health Services, Richmond, California 94804, USA. wprobert@dhs.ca.gov

Journal of Clinical Microbiology
|March 9, 2004
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...