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Ultraviolet sealing and poly(dimethylacrylamide) modification for poly(dimethylsiloxane)/glass microchips
1College of Chemistry and Chemical Engineering, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, P.R. China.
Electrophoresis
|March 9, 2004
Summary
UV irradiation and polymethylacrylamide (PDMA) coating create stable, hydrophilic poly(dimethylsiloxane)/glass microchips for capillary electrophoresis (CE). This method enables rapid separation of flavin compounds with high efficiency and reproducibility.
Area of Science:
- Analytical Chemistry
- Microfluidics
- Materials Science
Background:
- Poly(dimethylsiloxane) (PDMS)/glass microchips are widely used in capillary electrophoresis (CE).
- Surface modification is crucial for stable electroosmotic flow (EOF) and improved separation efficiency in CE microchips.
- Existing methods for PDMS surface modification can be complex or lack long-term stability.
Purpose of the Study:
- To develop simple and effective sealing methods for PDMS/glass CE microchips using UV irradiation.
- To investigate the use of polymethylacrylamide (PDMA) as a dynamic coating for modifying the inner surface of CE microchip channels.
- To evaluate the impact of PDMA coating on surface properties, EOF, and separation performance.
Main Methods:
- Fabrication of PDMS/glass microchips.
- Sealing of microchips using UV irradiation.
- Surface modification with polymethylacrylamide (PDMA) as a dynamic coating reagent.
- Surface characterization using atomic force microscopy (AFM), attenuated total reflection infrared (ATR-IR) spectroscopy, and contact angle measurements.
- Capillary electrophoresis separation of flavin compounds (flavin mononucleotide, flavin adenine dinucleotide, fluorescein).
Main Results:
- PDMA forms a stable coating on both PDMS and glass surfaces.
- The PDMA coating effectively eliminates non-homogeneous electroosmotic flow (EOF) in the microchip channels.
- PDMA coating improves the hydrophilicity of PDMS surfaces.
- Separation of flavin mixtures was achieved in 35 seconds with high efficiency (≥1365 theoretical plates) and good reproducibility (RSD < 4%).
Conclusions:
- UV-initiated sealing combined with PDMA dynamic coating provides a robust and efficient method for fabricating PDMS/glass CE microchips.
- The PDMA coating enhances microchip performance by stabilizing EOF and improving surface properties.
- This approach offers a promising platform for rapid and reproducible microchip electrophoresis analyses.