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DEC-205 expression on migrating dendritic cells in afferent lymph
Daniel R Gliddon1, Jayne C Hope, Gareth P Brooke
1Institute for Animal Health, Compton, Newbury, Berks. RG20 7NN, UK.
Immunology
|March 11, 2004
Summary
Bovine WC6 antigen is an orthologue of human DEC-205 (CD205), highly expressed on dendritic cells (DCs) in cattle lymph. This finding reveals heterogeneity within these bovine DCs, impacting immune cell research.
Area of Science:
- Immunology
- Cell Biology
- Veterinary Science
Background:
- A high molecular weight molecule (bovine WC6 antigen) is expressed on cattle dendritic cells (DCs) in afferent lymph and lymphoid tissues.
- Its expression pattern suggests it may be an orthologue of human DEC-205 (CD205).
Purpose of the Study:
- To determine if the bovine WC6 antigen is an orthologue of human DEC-205.
- To characterize the expression of bovine DEC-205 on immune cells in cattle.
Main Methods:
- Polymerase chain reaction amplification of bovine DEC-205 cDNA.
- COS-7 cell transfection and staining with WC6-specific monoclonal antibody.
- Two-colour flow cytometry of mononuclear cells from afferent lymph and blood.
Main Results:
- Bovine DEC-205 cDNA shares significant sequence identity with human and mouse orthologues.
- Transfected cells expressed a molecule recognized by the WC6 antibody, confirming WC6 as a DEC-205 orthologue.
- High DEC-205 expression was confirmed on dendritic cells (DCs) in afferent lymph, co-expressing DC-LAMP and MHC class II.
- Subpopulations within the DEC-205+ DC-LAMP+ population expressed mannose receptor or SIRPalpha.
Conclusions:
- Bovine WC6 antigen is confirmed as the orthologue of DEC-205 (CD205).
- All dendritic cells (DCs) in afferent lymph express high levels of DEC-205.
- The DEC-205+ dendritic cell population in afferent lymph is heterogeneous, not uniformly mature.