Related Experiment Videos
Human S-antigen: peptide determinant recognition in uveitis patients
Parul Tripathi1, Sandeep Saxena, Virendra S Yadav
1Department of Immunology, Sanjay Gandhi Post-Graduate Institute of Medical Sciences, Lucknow 226014, India.
Experimental and Molecular Pathology
|March 11, 2004
Summary
This study reveals that specific peptides from human S-antigen trigger T-lymphocyte responses in uveitis patients, indicating a key role for this protein region in the disease. These findings advance our understanding of posterior uveitis pathogenesis.
Area of Science:
- Ophthalmology
- Immunology
- Molecular Biology
Background:
- Uveitis, an inflammation of the uveal tract, is a significant cause of vision loss.
- Activated T lymphocytes are implicated in the progression of posterior uveitis.
Purpose of the Study:
- To investigate the proliferative response of lymphocytes from uveitis patients to retinal S-antigen peptides.
- To analyze surface marker expression and cytokine profiles in uveitis patients.
Main Methods:
- Lymphocyte proliferation assays using synthetic S-antigen peptides.
- Flow cytometry for detecting CD4, CD8, CD29, and CD45RA surface markers.
- Ribonuclease protection assay (RPA) for cytokine analysis in peripheral blood mononuclear cells (PBMCs).
Main Results:
- Nineteen of 32 uveitis patients exhibited lymphocyte proliferation against S-antigen or its peptides.
- A significant response was observed to peptides from the 231-270 amino acid region of S-antigen.
- Uveitis patients showed altered percentages of T-lymphocyte subsets (CD4+, CD8+, CD29+, CD45RA+) and elevated levels of specific cytokines (IL-5, IL-10, IL-15, IL-9, IL-2, IL-13, IFN-gamma) compared to controls.
Conclusions:
- Specific regions of S-antigen are involved in triggering immune responses in idiopathic uveitis.
- The study highlights the role of T-lymphocyte activation and specific cytokine profiles in the pathogenesis of posterior uveitis.