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Updated: Aug 25, 2026

Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy (VA-TIRFM)
Published on: October 2, 2012
Measuring a colloidal particle's interaction with a flat surface under nonequilibrium conditions. Total internal
S H Behrens1, J Plewa, D G Grier
1Dept. of Physics, James Franck Institute, and Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL 60637, USA.
Abstract:
A new and general approach is proposed to analyze the dynamics of a colloidal particle interacting with a nearby wall. This analysis can be used to determine the acting forces even when the system is non-stationary. As an illustration, we use total internal reflection microscopy to investigate the forces acting on a polystyrene sulfate latex particle as it is receding from a charged glass surface.
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