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Updated: Aug 25, 2026

Mass Spectrometric Analysis of Glycosphingolipid Antigens
Published on: April 16, 2013
Differentiation of isomeric oligosaccharide structures by ESI tandem MS and GC-MS
Lobvi E Matamoros Fernández1, Nicolai Obel, Henrik Vibe Scheller
1Department of Biochemistry and Molecular Biology, Institute of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark. lobvi@yahoo.com
Abstract:
A mixture of arabinoxylan oligosaccharides from wheat seedling was permethylated and analyzed by electrospray ion trap MS and GC-MS. The presence of isomeric structures differing in degree of branching and position of the branched residue along the xylose backbone was demonstrated for oligosaccharides with four and five monosaccharide residues. No isomeric structures were found for oligosaccharides with three monosaccharide residues. Linkage analysis by GC-MS reveals that xylose residues were substituted with single arabinoxyl residues at C-3.
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